Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, rat brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11361-1-AP targets Dynamitin (1-401 AA) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Dynamitin (1-401 AA) fusion protein Ag1902 相同性解析による交差性が予測される生物種 |
| Full Name | dynactin 2 (p50) |
| Calculated molecular weight | 401 aa, 44 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC014083 |
| Gene Symbol | Dynamitin |
| Gene ID (NCBI) | 10540 |
| RRID | AB_2090609 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13561 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83aa is responsible for calmodulin binding. This antibody is against the full length p50 dynamitin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Dynamitin (1-401 AA) antibody 11361-1-AP | Download protocol |
| IHC protocol for Dynamitin (1-401 AA) antibody 11361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |