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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Dynamitin (1-401 AA) Polyclonal antibody

Dynamitin (1-401 AA) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11361-1-AP

Synonyms

DCTN2, DYNAMITIN, Dynamitin (1-83 AA), Dynamitin (1-86 AA), Dynamitin (200-406 aa)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Dynamitin (1-401 AA) Polyclonal antibody (11361-1-AP)

    WB analysis using 11361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11361-1-AP (Dynamitin (1-401 AA) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Dynamitin (1-401 AA) Polyclonal antibody (11361-1-AP)

    IHC staining of human stomach cancer using 11361-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11361-1-AP (Dynamitin (1-401 AA) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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Tested Applications

Positive WB detected inA375 cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11361-1-AP targets Dynamitin (1-401 AA) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dynamitin (1-401 AA) fusion protein Ag1902 相同性解析による交差性が予測される生物種
Full Name dynactin 2 (p50)
Calculated molecular weight 401 aa, 44 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC014083
Gene symbol Dynamitin
Gene ID (NCBI) 10540
RRIDAB_2090609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83aa is responsible for calmodulin binding. This antibody is against the full length p50 dynamitin.

Protocols

Product Specific Protocols
WB protocol for Dynamitin (1-401 AA) antibody 11361-1-APDownload protocol
IHC protocol for Dynamitin (1-401 AA) antibody 11361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols