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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    WB analysis using 67428-1-Ig (same clone as 67428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67428-1-Ig (EIF4G2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    WB analysis using 67428-1-Ig (same clone as 67428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IHC staining of human breast cancer using 67428-1-Ig (same clone as 67428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IHC staining of human prostate cancer using 67428-1-Ig (same clone as 67428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IHC staining of human prostate cancer using 67428-1-Ig (same clone as 67428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67428-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IHC staining of human breast cancer using 67428-1-Ig (same clone as 67428-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67428-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IF Staining of HepG2 using 67428-1-Ig (same clone as 67428-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67428-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using eIF4G2/DAP5 Monoclonal antibody (67428-1-Ig)

    IF Staining of HeLa using 67428-1-Ig (same clone as 67428-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using eIF4G2/DAP5 antibody (67428-1-Ig, Clone: 3C11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67428-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

eIF4G2/DAP5 Monoclonal antibody, PBS Only

eIF4G2/DAP5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67428-1-PBS
Clone No.3C11A3

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, EIF4G2, FLJ41344, NAT1, p97

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67428-1-PBS targets eIF4G2/DAP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen eIF4G2/DAP5 fusion protein Ag12080 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4 gamma, 2
Calculated molecular weight 907 aa, 102 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC043149
Gene Symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2882667
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78344
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.