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  • Featured Product
  • KD/KO Validated

eRF3a/GSPT1 Monoclonal antibody, PBS Only

eRF3a/GSPT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A3H6

Cat no : 68217-1-PBS

Synonyms

eRF3a, GSPT1, G1 to S phase transition 1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, ETF3A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using eRF3a/GSPT1 Monoclonal antibody (68217-1-Ig)

    WB analysis using 68217-1-Ig (same clone as 68217-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68217-1-Ig (eRF3a/GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68217-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using eRF3a/GSPT1 Monoclonal antibody (68217-1-Ig)

    WB analysis of COLO 320 using 68217-1-Ig (same clone as 68217-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 68217-1-Ig (GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68217-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using eRF3a/GSPT1 Monoclonal antibody (68217-1-Ig)

    WB analysis of pig brain using 68217-1-Ig (same clone as 68217-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68217-1-Ig (GSPT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68217-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using eRF3a/GSPT1 Monoclonal antibody (68217-1-Ig)

    WB analysis of HepG2 using 68217-1-Ig (same clone as 68217-1-PBS)

    WB result of eRF3a/GSPT1 antibody (68217-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-eRF3a/GSPT1 transfected HepG2 cells. This data was developed using the same antibody clone with 68217-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using eRF3a/GSPT1 Monoclonal antibody (68217-1-Ig)

    IF Staining of PC-3 using 68217-1-Ig (same clone as 68217-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using eRF3a/GSPT1 antibody (68217-1-Ig, Clone: 2A3H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68217-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using eRF3a/GSPT1 Monoclonal antibody (68217-1-Ig)

    IF Staining of HepG2 using 68217-1-Ig (same clone as 68217-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using eRF3a/GSPT1 antibody (68217-1-Ig, Clone: 2A3H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68217-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68217-1-PBS targets eRF3a/GSPT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen eRF3a/GSPT1 fusion protein Ag27986 相同性解析による交差性が予測される生物種
Full Name G1 to S phase transition 1
Calculated molecular weight 68 aa, 4 kDa
Observed molecular weight80-85 kDa
GenBank accession numberBC009503
Gene symbol GSPT1
Gene ID (NCBI) 2935
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. eRF3a, also known as GSPT1, is one subunit of eRF3(PMID:15917414,12923185). It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. eRF3a/GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of eRF3a/GSPT1 as a BIR3-binding protein-Using a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1/eRF3a protein, lacking the first 69 residues(PMID: 12865429).