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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

fetuin-B Polyclonal antibody

fetuin-B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18052-1-AP

Synonyms

16G2, FETUB, fetuin B, Fetuin like protein IRL685, fetuin-B, Gugu, IRL685

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using fetuin-B Polyclonal antibody (18052-1-AP)

    WB analysis of human plasma using 18052-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using fetuin-B Polyclonal antibody (18052-1-AP)

    WB analysis of human plasma using 18052-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using fetuin-B Polyclonal antibody (18052-1-AP)

    IP experiment of human plasma using 18052-1-AP

    IP result of anti-fetuin-B (IP:18052-1-AP, 4ug; Detection:18052-1-AP 1:2000) with human plasma lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using fetuin-B Polyclonal antibody (18052-1-AP)

    IHC staining of human liver cancer using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using fetuin-B Polyclonal antibody (18052-1-AP)

    IHC staining of human liver cancer using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using fetuin-B Polyclonal antibody (18052-1-AP)

    IHC staining of mouse liver using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using fetuin-B Polyclonal antibody (18052-1-AP)

    IHC staining of mouse liver using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman blood tissue, human plasma
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18052-1-AP targets fetuin-B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fetuin-B fusion protein Ag12562 相同性解析による交差性が予測される生物種
Full Name fetuin B
Calculated molecular weight 382 aa, 42 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC074734
Gene symbol Fetuin B
Gene ID (NCBI) 26998
RRIDAB_2103181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fetuin-B is a protein that in humans is encoded by the FETUB gene. The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the ins and hepatocyte growth factor receptors, and response to systemic inflammation. It may be a protease inhibitor required for egg fertilization.

Protocols

Product Specific Protocols
WB protocol for fetuin-B antibody 18052-1-APDownload protocol
IHC protocol for fetuin-B antibody 18052-1-APDownload protocol
IP protocol for fetuin-B antibody 18052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols