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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

fetuin-B Monoclonal antibody

fetuin-B Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rabbit and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H7D11

Cat no : 67002-1-Ig

Synonyms

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

Validation Data Gallery

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  • Western Blot (WB) analysis of human blood using fetuin-B Monoclonal antibody (67002-1-Ig)

    WB analysis of human blood using 67002-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using fetuin-B Monoclonal antibody (67002-1-Ig)

    WB analysis of human plasma using 67002-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit serum using fetuin-B Monoclonal antibody (67002-1-Ig)

    WB analysis of rabbit serum using 67002-1-Ig

    rabbit serum were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using fetuin-B Monoclonal antibody (67002-1-Ig)

    IHC staining of human liver using 67002-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using fetuin-B Monoclonal antibody (67002-1-Ig)

    IHC staining of human liver using 67002-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using fetuin-B Monoclonal antibody (67002-1-Ig)

    IF Staining of human liver cancer using 67002-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma, human blood, rabbit serum
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

67002-1-Ig targets fetuin-B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rabbit samples.

Tested Reactivity human, rabbit
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen fetuin-B fusion protein Ag12463 相同性解析による交差性が予測される生物種
Full Name fetuin B
Calculated molecular weight 382 aa, 42 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC074734
Gene symbol Fetuin B
Gene ID (NCBI) 26998
RRIDAB_2882319
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

Protocols

Product Specific Protocols
WB protocol for fetuin-B antibody 67002-1-IgDownload protocol
IHC protocol for fetuin-B antibody 67002-1-IgDownload protocol
IF protocol for fetuin-B antibody 67002-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Agric Food Chem

Baicalein Enhances the Effect of Acarbose on the Improvement of Nonalcoholic Fatty Liver Disease Associated with Prediabetes via the Inhibition of De Novo Lipogenesis.

Authors - Yan Xing
View Article
mouseWB

J Proteome Res

Novel Regulators of Retina Neovascularization: A Proteomics Approach.

Authors - Manhong Xu
View Article
mouseWB

Front Pharmacol

Delineation of renal protein profiles in aristolochic acid I-induced nephrotoxicity in mice by label-free quantitative proteomics

Authors - Xinhui Liu
View Article