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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FLNC Polyclonal antibody

FLNC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28492-1-AP

Synonyms

FLN2, FilaminC, Filamin C, Filamin 2, ABPL

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of SGC-7901 cells using FLNC Polyclonal antibody (28492-1-AP)

    WB analysis of SGC-7901 using 28492-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 28492-1-AP (filamin-c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon using FLNC Polyclonal antibody (28492-1-AP)

    IHC staining of human colon using 28492-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 28492-1-AP (FLNC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28492-1-AP targets FLNC in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLNC fusion protein Ag29328 相同性解析による交差性が予測される生物種
Full Name filamin C, gamma (actin binding protein 280)
Calculated molecular weight 291 kDa
Observed molecular weight 280-300 kDa
GenBank accession numberNM_001458
Gene symbol FLNC
Gene ID (NCBI) 2318
RRIDAB_2918171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Filamin C (FLNC; also known as γ-FLN; ABP-L; FLN2), a muscle-specific filamin and a large actin-cross-linking protein. Human filamins are ~280 kDa homodimers, each monomer consisting of an N-terminal actin-binding domain followed by 24 immunoglobulin (Ig)-like domains (d1 to d24), the last of which mediates dimerization. FLNC is specifically expressed in cardiomyocytes and skeletal myocytes and is involved in the maintenance of structural integrity. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. ( PMID: 25577646, PMID: 30867563, PMID: 31131323)

Protocols

Product Specific Protocols
WB protocol for FLNC antibody 28492-1-APDownload protocol
IHC protocol for FLNC antibody 28492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions

Authors - Ilenia Matera
View Article