• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

gp130/IL6ST Monoclonal antibody, PBS Only

gp130/IL6ST Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7C5

Cat no : 67766-1-PBS

Synonyms

IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    WB analysis using 67766-1-Ig (same clone as 67766-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    WB analysis of NCCIT using 67766-1-Ig (same clone as 67766-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human tonsillitis using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human tonsillitis using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human colon cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human colon cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human liver cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human liver cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human lung cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using gp130/IL6ST Monoclonal antibody (67766-1-Ig)

    IHC staining of human lung cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67766-1-PBS targets gp130/IL6ST in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen gp130/IL6ST fusion protein Ag28808 相同性解析による交差性が予測される生物種
Full Name interleukin 6 signal transducer (gp130, oncostatin M receptor)
Calculated molecular weight 104 kDa
Observed molecular weight130-140 kDa
GenBank accession numberNM_002184
Gene symbol IL6ST
Gene ID (NCBI) 3572
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).