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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

gp130 Monoclonal antibody, PBS Only

gp130 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7C5

Cat no : 67766-1-PBS

Synonyms

CD130, CDw130, Glycoprotein 130, GP130, GP130 RAPS, IL 6 receptor subunit beta, IL 6R beta, IL 6R subunit beta, IL 6RB, IL6R beta, IL6ST, Membrane glycoprotein 130

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using gp130 Monoclonal antibody (67766-1-Ig)

    WB analysis using 67766-1-Ig (same clone as 67766-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using gp130 Monoclonal antibody (67766-1-Ig)

    WB analysis of NCCIT using 67766-1-Ig (same clone as 67766-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human placenta using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human colon cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human colon cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human liver cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human liver cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human lung cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human lung cancer using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human tonsillitis using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using gp130 Monoclonal antibody (67766-1-Ig)

    IHC staining of human tonsillitis using 67766-1-Ig (same clone as 67766-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67766-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67766-1-PBS targets gp130 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen gp130 fusion protein Ag28808 相同性解析による交差性が予測される生物種
Full Name interleukin 6 signal transducer (gp130, oncostatin M receptor)
Calculated molecular weight 104 kDa
Observed molecular weight130-140 kDa
GenBank accession numberNM_002184
Gene symbol IL6ST
Gene ID (NCBI) 3572
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.