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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using gp130/IL6ST Recombinant antibody (84794-4-RR)

    WB analysis using 84794-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84794-4-RR (gp130/IL6ST antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84794-4-RR

    Affinity and Kinetic Characterization of 84794-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84794-4-RR against Mouse gp130/IL6ST were performed. The affinity constant is 89.7 pM.

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gp130/IL6ST Recombinant antibody

gp130/IL6ST Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84794-4-RR
Clone No.242236E1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

IL-6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130, CD130

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inNIH/3T3 cells, Neuro-2a cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84794-4-RR targets gp130/IL6ST in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 6 signal transducer
Calculated molecular weight102kDa, 917aa
Observed molecular weight140 kDa
GenBank accession numberNM_010560.3
Gene Symbol Il6st
Gene ID (NCBI) 16195
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ00560
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GP130, also known as IL6ST, is a transmembrane protein that serves as a shared signal-transducing subunit for a variety of cytokines(PMID: 39414364), including interleukin-6 (IL-6), IL-11, IL-27, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC)/CLC factor 1 (CLCF-1), and neuropoietin (PMID: 24099136). This protein is highly conserved among vertebrates and plays a crucial role in mediating interactions between a wide range of immune and non-immune cells. GP130 was originally discovered in 1978 and was successfully cloned and expressed in 1990, leading to a deeper understanding of cytokine signaling pathways (PMID: 308233).

Protocols

Product Specific Protocols
WB protocol for gp130/IL6ST antibody 84794-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols