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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human gp130/IL6ST Rabbit Recombinant Antibody

gp130/IL6ST Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240992H1

Cat no : 98121-1-RR

Synonyms

IL6ST, IL 6 receptor subunit beta, GP130 RAPS, GP130, Glycoprotein 130

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR)

    FC experiment of human peripheral blood leukocytes using 98121-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR, Clone: 240992H1) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR)

    FC experiment of human peripheral blood leukocytes using 98121-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human gp130/IL6ST Rabbit Recombinant Antibody (98121-1-RR, Clone: 240992H1) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98121-1-RR

    Affinity and Kinetic Characterization of 98121-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98121-1-RR against Human gp130/IL6ST were performed. The affinity constant is 115 nM.

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Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98121-1-RR is gp130/IL6ST Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 6 signal transducer (gp130, oncostatin M receptor)
Calculated molecular weight 104 kDa
GenBank accession numberNM_002184.3
Gene symbol IL6ST
Gene ID (NCBI) 3572
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3.

Protocols

Product Specific Protocols
FC protocol for gp130/IL6ST antibody 98121-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols