GST Tag Polyclonal antibody
GST Tag Polyclonal Antibody for ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (3)
Applications
WB, IF, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
218
Cat no : 10000-0-AP
Synonyms
"GST Tag Antibodies" Comparison
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Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 147 publications below |
| IF | See 3 publications below |
| IP | See 24 publications below |
| ELISA | See 1 publications below |
| CoIP | See 5 publications below |
| ChIP | See 2 publications below |
Product Information
10000-0-AP targets GST Tag in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | GST Tag |
| Calculated molecular weight | 26 kDa |
| GenBank accession number | FN315687 |
| Gene symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11042316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis | ||
Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis | ||
Ann Rheum Dis Autoantibodies identify primary Sjögren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B | ||
Immunity Targeting 7-Dehydrocholesterol Reductase Integrates Cholesterol Metabolism and IRF3 Activation to Eliminate Infection. | ||
Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins | ||
Nat Struct Mol Biol CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Daniel (Verified Customer) (06-10-2022) | We use this anti-GST antibody for GST fusion protein-lipid interactions. We found this antibody gives much less background than other antibodies.
 |
FH Lana (Verified Customer) (04-06-2022) | SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate, 3 – GST protein, 4 – GST fusion protein
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FH Shihab (Verified Customer) (10-28-2021) | Tried this antibody for a control experiment and worked nicely. Will be continuing to try this in immunofluorescence
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FH Amie (Verified Customer) (07-27-2021) | I compared this antibody directly to the proteintech mouse monoclonal (66001-2-Ig). In the provided image, the first 4 lanes of each gel are identical. The rabbit antibody seems to be slightly better (less background) than the mouse.
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FH Yan (Verified Customer) (02-21-2021) | It worked very well when I performed western blot after immunoprecipitation.
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