hCG Alpha Polyclonal antibody
hCG Alpha Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25014-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | JAR cells, human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
25014-1-AP targets hCG Alpha in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | hCG Alpha fusion protein Ag17313 相同性解析による交差性が予測される生物種 |
| Full Name | glycoprotein hormones, alpha polypeptide |
| Calculated molecular weight | 116 aa, 13 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC020782 |
| Gene symbol | hCG Alpha |
| Gene ID (NCBI) | 1081 |
| RRID | AB_2879846 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for hCG Alpha antibody 25014-1-AP | Download protocol |
| IHC protocol for hCG Alpha antibody 25014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Endocrinol Metab A Patient with a Thyrotropin-secreting Microadenoma and Resistance to Thyroid Hormone (P453T). | ||
iScience L3MBTL2-mediated CGA transcriptional suppression promotes pancreatic cancer progression through modulating autophagy. | ||
Front Endocrinol (Lausanne) Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment |
