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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of HSC-T6 using 66989-1-Ig (same clone as 66989-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of NIH/3T3 using 66989-1-Ig (same clone as 66989-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    WB analysis of mouse liver using 66989-1-Ig (same clone as 66989-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using hIST1 Monoclonal antibody (66989-1-Ig)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using hIST1 Monoclonal antibody (66989-1-Ig)

    IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

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hIST1 Monoclonal antibody, PBS Only

hIST1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66989-1-PBS
Clone No.1E12A7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66989-1-PBS targets hIST1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen hIST1 fusion protein Ag28364 相同性解析による交差性が予測される生物種
Full Name KIAA0174
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC000430
Gene Symbol hIST1
Gene ID (NCBI) 9798
RRIDAB_2882306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53990
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.