Featured Product
KD/KO Validated

hnRNP-E1 Polyclonal antibody

Name: hnRNP-E1 Polyclonal antibody
Brand: Proteintech
SKU: 14523-1-AP
Rating: 5.00 (1 reviews)

hnRNP-E1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 14523-1-AP

Synonyms

hnRNP E1/PCBP1, hnRNP E1, PCBP1, Nucleic acid-binding protein SUB2.3, HNRPE1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 14523-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14523-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14523-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14523-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14523-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 14523-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14523-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14523-1-AP (hnRNP-E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14523-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of NIH/3T3 using 14523-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of A431 using 14523-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 14523-1-AP
Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using hnRNP-E1 antibody (14523-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

-/ -

Cat No. 14523-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, MCF-7 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, NIH/3T3 cells, A431 cells, sodium arsenite treated HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

14523-1-AP targets hnRNP-E1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	hnRNP-E1 fusion protein Ag6007 相同性解析による交差性が予測される生物種
Full Name	poly(rC) binding protein 1
Calculated molecular weight	37 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC039742
Gene symbol	PCBP1
Gene ID (NCBI)	5093
RRID	AB_2160619
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

hnRNP-E1 (heterogeneous nuclear ribonucleoprotein E1) is also named as PCBP1 and Alpha-CP1. Poly(rC)-binding protein 1 (PCBP1 also called hnRNP E1) belongs to a hnRNP family that is composed of hnRNP K/J and the alpha-complex proteins (PCBP1-4α or CP1-4). Proteins in this family contain three hnRNP K homology (KH) domains for RNA-binding. The human PCBP1 gene encodes the hnRNP E1 protein, and was initially defined as clone sub2.3, with poly(C)-binding activity and observed similarity to hnRNP E2 (PMID: 8152927). hnRNP-E1 is an RNA binding protein that regulates diverse processes including translation and splicing (PMID:29396052). hnRNP E1 regulates splicing of EMT-associated genes including CD44 and PNUTS (PMID: 28825698). This antibody recognizes hnRNP E1/E2.

Protocols

Product Specific Protocols
WB protocol for hnRNP-E1 antibody 14523-1-AP	Download protocol
IHC protocol for hnRNP-E1 antibody 14523-1-AP	Download protocol
IF protocol for hnRNP-E1 antibody 14523-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	J Clin Invest Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. Authors - Xue Gong View Article
human	IF	Nucleic Acids Res G-Quadruplex binding enantiomers show chiral selective interactions with human telomere. Authors - Jiasi Wang View Article
human	IHC	Bioengineered PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma. Authors - Kaiming Peng View Article
rat	WB,IHC	Pathol Int PCBP2 knockdown promotes ferroptosis in malignant mesothelioma. Authors - Lin Yue KD Validated View Article
mouse	WB	Mol Cell Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X. Authors - David Colognori View Article
human	WB	Cell Human hematopoietic stem cell vulnerability to ferroptosis Authors - Jiawei Zhao View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

S. H. (Verified Customer) (02-24-2020)

we are happy for the result we got.

Applications: Western Blot,
Primary Antibody Dilution: 1000x dilution
Cell Tissue Type: 293T CELLS

hnRNP-E1 Antibody Western Blot, validation (1000x dilution dilution) in 293T CELLS (Cat no:14523-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

hnRNP-E1 Polyclonal antibody

hnRNP-E1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 14523-1-AP

WB analysis of HeLa using 14523-1-AP

WB analysis of K-562 using 14523-1-AP

WB analysis of MCF-7 using 14523-1-AP

WB analysis of HepG2 using 14523-1-AP

IHC staining of human lung cancer using 14523-1-AP

IHC staining of human lung cancer using 14523-1-AP

IF Staining of HepG2 using 14523-1-AP

IF Staining of NIH/3T3 using 14523-1-AP

IF Staining of A431 using 14523-1-AP

IF Staining of HeLa using 14523-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

G-Quadruplex binding enantiomers show chiral selective interactions with human telomere.

PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma.

PCBP2 knockdown promotes ferroptosis in malignant mesothelioma.

Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.

Human hematopoietic stem cell vulnerability to ferroptosis

Reviews