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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

human IgM Polyclonal antibody, PBS Only

human IgM Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11016-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using human IgM Polyclonal antibody (11016-1-AP)

    WB analysis of human plasma using 11016-1-AP (same clone as 11016-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 11016-1-AP (IGHM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Polyclonal antibody (11016-1-AP)

    IHC staining of human tonsillitis using 11016-1-AP (same clone as 11016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Polyclonal antibody (11016-1-AP)

    IHC staining of human tonsillitis using 11016-1-AP (same clone as 11016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11016-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11016-1-PBS targets human IgM in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant mu
Calculated molecular weight 69 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC009851
Gene symbol IGHM
Gene ID (NCBI) 3507
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa).