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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

human IgM Monoclonal antibody, PBS Only

human IgM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D10B10

Cat no : 66484-1-PBS

Synonyms

IgM, IGHM, VH, MU, 2D10B10

Validation Data Gallery

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  • Western Blot (WB) analysis of Human plasma using human IgM Monoclonal antibody (66484-1-Ig)

    WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

    0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Human plasma using human IgM Monoclonal antibody (66484-1-Ig)

    WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

    0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using human IgM Monoclonal antibody (66484-1-Ig)

    IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66484-1-Ig (IGHM antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66484-1-PBS targets human IgM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human IgM fusion protein Ag1459 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant mu
Calculated molecular weight 69 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC009851
Gene symbol IGHM
Gene ID (NCBI) 3507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).