Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)
0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)
0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:3200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (human IgM antibody) at dilution of 1:3200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66484-1-Ig (IGHM antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66484-1-PBS in a different storage buffer formulation.

human IgM Monoclonal antibody, PBS Only

human IgM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66484-1-PBS

Clone No.2D10B10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

IgM, IGHM, VH, MU, 2D10B10

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66484-1-PBS targets human IgM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	human IgM fusion protein Ag1459 相同性解析による交差性が予測される生物種
Full Name	immunoglobulin heavy constant mu
Calculated molecular weight	69 kDa
Observed molecular weight	75 kDa
GenBank accession number	BC009851
Gene Symbol	IGHM
Gene ID (NCBI)	3507
RRID	AB_2881850
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

WB analysis of Human plasma using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IHC staining of human lymphoma using 66484-1-Ig (same clone as 66484-1-PBS)

IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

IF Staining of human appendicitis using 66484-1-Ig (same clone as 66484-1-PBS)

IF Staining of human tonsillitis using 66484-1-Ig (same clone as 66484-1-PBS)

human IgM Monoclonal antibody, PBS Only

human IgM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information