Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10298-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10298-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:35000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10298-1-AP
WB result of Lamin A/C antibody (10298-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells.
WB analysis of A375 using 10298-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10298-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 10298-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 10298-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 10298-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10298-1-AP
IP result of anti-Lamin A/C (IP:10298-1-AP, 4ug; Detection:10298-1-AP 1:50000) with HeLa cells lysate 1360 ug.
IP experiment of A375 using 10298-1-AP
IP result of anti-Lamin A/C (IP:10298-1-AP, 3ug; Detection:10298-1-AP 1:1000) with A375 cells lysate 800ug.
IHC staining of human normal colon using 10298-1-AP
Immunohistochemical analysis of paraffin-embedded normal colon slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:7000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 10298-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 10298-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10298-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10298-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin A/C antibody (10298-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 10298-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10298-1-AP
Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .
FC experiment of HEK-293T using 10298-1-AP
1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Lamin A/C (10298-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

Lamin A/C Polyclonal antibody

Lamin A/C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10298-1-AP

Publications(245)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

LaminA/C, lamin A, Prelamin-A/C, LMNA, LMN1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration: 150 μL, 700 μg/ml

150 μL, 700 μg/ml

¥36,000/ 150 μL

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Overview

Tested Applications
Product Information
Publications (245)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HEK-293 cells, HUVEC cells, mouse ovary tissue, SKOV-3 cells, HeLa cells, A375 cells, C6 cells, NIH/3T3 cells
Positive IP detected in	HeLa cells, A375 cells
Positive IHC detected in	mouse heart tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells
Positive FC (Intra) detected in	HEK-293T cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 227 publications below
IHC	See 2 publications below
IF	See 14 publications below
IP	See 4 publications below

Product Information

10298-1-AP targets Lamin A/C in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Lamin A/C fusion protein Ag0408 相同性解析による交差性が予測される生物種
Full Name	lamin A/C
Calculated molecular weight	65 kDa
Observed molecular weight	65 kDa, 70 kDa
GenBank accession number	BC003162
Gene Symbol	Lamin A/C
Gene ID (NCBI)	4000
ENSEMBL Gene ID	ENSG00000160789
RRID	AB_2296961
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02545
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lamin A/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure, and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa, and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

Protocols

Product Specific Protocols
WB protocol for Lamin A/C antibody 10298-1-AP	Download protocol
IHC protocol for Lamin A/C antibody 10298-1-AP	Download protocol
IF protocol for Lamin A/C antibody 10298-1-AP	Download protocol
IP protocol for Lamin A/C antibody 10298-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer Authors - Rashmi Bharti View Article
human	IF	Nat Microbiol Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus. Authors - Adarsh Dharan View Article
human	WB	Mol Cell Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells. Authors - Jia Xiong View Article
human	WB	J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. Authors - Xiaohan Gao View Article
human	WB	Nat Chem Biol E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells Authors - Siqi Shen View Article
human	WB	Cancer Cell SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. Authors - Lan Fang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10298-1-AP

WB analysis using 10298-1-AP

WB analysis of HeLa using 10298-1-AP

WB analysis of A375 using 10298-1-AP

WB analysis of HEK-293 using 10298-1-AP

WB analysis of mouse ovary using 10298-1-AP

WB analysis of SKOV-3 using 10298-1-AP

WB analysis of C6 using 10298-1-AP

IP experiment of HeLa using 10298-1-AP

IP experiment of A375 using 10298-1-AP

IHC staining of human normal colon using 10298-1-AP

IHC staining of mouse heart using 10298-1-AP

IHC staining of mouse heart using 10298-1-AP

IF Staining of HepG2 using 10298-1-AP

IF Staining of HepG2 using 10298-1-AP

IF Staining of HepG2 using 10298-1-AP

IF Staining of HeLa using 10298-1-AP

FC experiment of HEK-293T using 10298-1-AP

Recommended products:

Multi-rAb Recombinant Secondary Antibodies

New FlexAble 2.0 Antibody Labeling Kits

Lamin A/C Polyclonal antibody

Lamin A/C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

¥36,000/ 150 μL

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.

Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells

SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.

^NewFlexAble 2.0 Antibody Labeling Kits