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5-methylcytosine Monoclonal antibody

5-methylcytosine Monoclonal Antibody for IHC, Dot Blot, ELISA
Cat No. 68301-1-Ig
Clone No.2C9G9

Host / Isotype

Mouse / IgG2b

Reactivity

5mc, chemical compound, m5c and More (2)

Applications

WB, IHC, IF, Dot Blot, ELISA

m5C, 5mC

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IHC detected inmouse testis tissue, rat brain tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive ELISA detected incompound
Positive Dot Blot detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
DOT BLOTDOT BLOT : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68301-1-Ig targets 5-methylcytosine in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with 5mc, chemical compound, m5c samples.

Tested Reactivity 5mc, chemical compound, m5c
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Compound 相同性解析による交差性が予測される生物種
Full Name 5-methylcytosine
Gene Symbol
Gene ID (NCBI)
RRIDAB_2935380
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216). This antibody can recognize both 5mC in DNA and m5C in RNA.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf

Protocols

Product Specific Protocols
IHC protocol for 5-methylcytosine antibody 68301-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cancer Lett

NSUN4 mediated RNA 5-methylcytosine promotes the malignant progression of glioma through improving the CDC42 mRNA stabilization

Authors - Zhen Zhao
human

Biomedicines

NSUN2 Promotes Head and Neck Squamous Cell Carcinoma Progression by Targeting EMT-Related Gene LAMC2 in an m5C-YBX1-Dependent Manner

Authors - Shuojin Huang
humanWB

Redox Biol

NSUN2 lactylation drives cancer cell resistance to ferroptosis through enhancing GCLC-dependent glutathione synthesis

Authors - Kaifeng Niu
mouseIF

Eur J Pharmacol

5- methylcytidine effectively improves spermatogenesis recovery in busulfan-induced oligoasthenospermia mice

Authors - Chengniu Wang
human

Cancer Biol Ther

Function of m5C RNA methyltransferase NOP2 in high-grade serous ovarian cancer

Authors - Shimin Yang
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