5-methylcytosine Monoclonal antibody
5-methylcytosine Monoclonal Antibody for IHC, Dot Blot, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
chemical compound, m5c and More (2)
Applications
IHC, IF, Dot Blot, ELISA
Conjugate
Unconjugated
CloneNo.
2C9G9
Cat no : 68301-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse testis tissue, rat brain tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive ELISA detected in | compound |
| Positive Dot Blot detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| DOT BLOT | DOT BLOT : 1:2500-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
68301-1-Ig targets 5-methylcytosine in IHC, IF, Dot Blot, ELISA applications and shows reactivity with chemical compound, m5c samples.
| Tested Reactivity | chemical compound, m5c |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Compound 相同性解析による交差性が予測される生物種 |
| Full Name | 5-methylcytosine |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
In eukaryotes, 5-methylcytosine in DNA (5mC) and its oxidized derivatives (5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)) are the most prominent modifications and have been suggested to contribute to epigenetic gene regulation through a variety of different mechanisms. m5C has also been found in mRNA, rRNA and tRNA of representative organisms from all kinds of species. As reversible epigenetic modifications, m5C modifications of RNA affect the fate of the modified RNA molecules and play important roles in various biological processes including RNA stability control, protein synthesis, and transcriptional regulation (PMID: 35365216).
This antibody can recognize both 5mC in DNA and m5C in RNA.
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68301-1-IgDotBlot.pdf
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for 5-methylcytosine antibody 68301-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett NSUN4 mediated RNA 5-methylcytosine promotes the malignant progression of glioma through improving the CDC42 mRNA stabilization | ||
Eur J Pharmacol 5- methylcytidine effectively improves spermatogenesis recovery in busulfan-induced oligoasthenospermia mice | ||
Cancer Biol Ther Function of m5C RNA methyltransferase NOP2 in high-grade serous ovarian cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH DONGHONG (Verified Customer) (07-02-2024) | I used this antibody for the competitive elisa kit. Coating BSA-m5C and blocking the 96-well plate. Use the the standard to competitive bind m5C antibody. However, the standard curve was not good as m5C from Abcam (ab10805).
|
