Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68302-1-Ig (m7G antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68302-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68302-1-Ig (m7G antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68302-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68302-1-Ig (m7G antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68302-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68302-1-Ig (m7G antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68302-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68302-1-Ig (m7G antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68302-1-PBS in a different storage buffer formulation.

Indirect ELISA and competitive ELISA results show that this antibody is specific to m7G. Indirect ELISA (blue curve, refer to top X-right Y axis) was performed by coating BSA conjugated m7G at 10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of m7G or its structure analogue compound Guanosine was mixed in 20ng/mL primary antibody (red curve, refer to bottom X-left Y axis). This data was developed using the same antibody clone with 68302-1-PBS in a different storage buffer formulation.

Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with m7G antibody 68302-1-Ig at 1:5000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2b isotype control Monoclonal antibody 66360-3-Ig at the same dose. This data was developed using the same antibody clone with 68302-1-PBS in a different storage buffer formulation.