ChromoTek mEos-Trap Agarose Kit

The ChromoTek mEos-Trap Agarose Kit consists of an anti-mEos Nanobody/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mEos-tagged proteins from cell extracts of various organisms.

Specificity

mEos-Tag (mEos2 and mEos4b)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Cat no : metak

Synonyms

mEos Nanobody, mEos2, mEos4b



Product Information

The ChromoTek mEos-Trap Agarose Kit consists of an anti-mEos Nanobody/VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mEos-tagged proteins from cell extracts of various organisms.

DescriptionThe HA-Trap Agarose Kit contains mEos-Trap agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of mEos-tagged proteins.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, yeast, etc.

ApplicationsIP, Co-IP
Specificity/TargetBinds specifically to members of the mEos-tag family fused to a protein of interest at N-, C- or internal position. To ensure broad coverage of mEos variants, the Nanobody was optimized for binding to both mEos2 and mEos4b, which are representative of the different lineages of the mEos family. There is no cross-reactivity to other fluorescent proteins such as eGFP.
Binding Capacity15 ug of recombinant mEos4b (28 kDa) per 25 uL bead slurry
ConjugateAgarose beads; ~90 um (cross-linked 4% agarose beads)
Elution buffer2x SDS-sample buffer (Lammli)
Wash Buffer Compatibility2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.2% SDS, 1 M Urea
TypeNanobody
ClassRecombinant
HostCamelid
Affinity (KD) 75 nM
Compatibility with mass spectrometryThe mEos-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3552227
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

Component Description
mEos-Trap Agarose20 reactions (500 µl)
Lysis bufferOptimized for cytoplasmic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution