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  • Featured Product
  • KD/KO Validated

mTOR Recombinant antibody, PBS Only

mTOR Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6H23

Cat no : 81670-1-PBS

Synonyms

FRAP1, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using mTOR Recombinant antibody (81670-1-RR)

    WB analysis using 81670-1-RR (same clone as 81670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81670-1-RR (mTOR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81670-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using mTOR Recombinant antibody (81670-1-RR)

    WB analysis of HeLa using 81670-1-RR (same clone as 81670-1-PBS)

    WB result of mTOR antibody (81670-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells. This data was developed using the same antibody clone with 81670-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using mTOR Recombinant antibody (81670-1-RR)

    IP experiment of HeLa using 81670-1-RR (same clone as 81670-1-PBS)

    IP result of anti-mTOR (IP:81670-1-RR, 4ug; Detection:81670-1-RR 1:1000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 81670-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using mTOR Recombinant antibody (81670-1-RR)

    IHC staining of human colon cancer using 81670-1-RR (same clone as 81670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81670-1-RR (mTOR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81670-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using mTOR Recombinant antibody (81670-1-RR)

    IF Staining of HepG2 using 81670-1-RR (same clone as 81670-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (81670-1-RR, Clone: 6H23 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81670-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using mTOR Recombinant antibody (81670-1-RR)

    IF Staining of HepG2 using 81670-1-RR (same clone as 81670-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using mTOR antibody (81670-1-RR, Clone: 6H23 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81670-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using mTOR Recombinant antibody (81670-1-RR)

    FC experiment of HeLa using 81670-1-RR (same clone as 81670-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human mTOR (81670-1-RR, Clone:6H23) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81670-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81670-1-RR

    Affinity and Kinetic Characterization of 81670-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81670-1-RR against Human mTOR were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81670-1-PBS targets mTOR in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen mTOR fusion protein Ag28395 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
Observed molecular weight250-289 kDa
GenBank accession numberNM_004958
Gene symbol mTOR
Gene ID (NCBI) 2475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

mTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.