- Featured Product
- KD/KO Validated
mTOR Recombinant antibody, PBS Only
mTOR Recombinant Antibody for Indirect ELISA, IP, IF/ICC, IHC, WB
Host / Isotype
Rabbit / IgG
Reactivity
rat, mouse, human
Applications
Indirect ELISA, IP, IF/ICC, IHC, WB
Conjugate
Unconjugated
CloneNo.
6H23
Cat no : 81670-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
81670-1-PBS targets mTOR in Indirect ELISA, IP, IF/ICC, IHC, WB applications and shows reactivity with rat, mouse, human samples.
Tested Reactivity | rat, mouse, human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | mTOR fusion protein Ag28395 相同性解析による交差性が予測される生物種 |
Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
Calculated molecular weight | 289 kDa |
Observed molecular weight | 250-289 kDa |
GenBank accession number | NM_004958 |
Gene symbol | mTOR |
Gene ID (NCBI) | 2475 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
mTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.