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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using nNOS Polyclonal antibody (18984-1-AP)

    IHC staining of mouse heart using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using nNOS Polyclonal antibody (18984-1-AP)

    IHC staining of mouse heart using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using nNOS Polyclonal antibody (18984-1-AP)

    IHC staining of rat testis using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using nNOS Polyclonal antibody (18984-1-AP)

    IHC staining of mouse brain using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using nNOS Polyclonal antibody (18984-1-AP)

    IHC staining of rat testis using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using nNOS Polyclonal antibody (18984-1-AP)

    IHC staining of mouse kidney using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using nNOS Polyclonal antibody (18984-1-AP)

    IHC staining of mouse kidney using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using nNOS Polyclonal antibody (18984-1-AP)

    IHC staining of mouse brain using 18984-1-AP (same clone as 18984-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using nNOS Polyclonal antibody (18984-1-AP)

    IF Staining of mouse brain using 18984-1-AP (same clone as 18984-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using nNOS antibody (18984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using nNOS Polyclonal antibody (18984-1-AP)

    IF Staining of mouse brain using 18984-1-AP (same clone as 18984-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using nNOS antibody (18984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18984-1-PBS in a different storage buffer formulation.

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nNOS Polyclonal antibody, PBS Only

nNOS Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 18984-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, Indirect ELISA

Synonyms

NOS1, N-NOS, NC-NOS, NC NOS, N NOS

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18984-1-PBS targets nNOS in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nitric oxide synthase 1 (neuronal)
Calculated molecular weight 161 kDa
GenBank accession numberNM_000620
Gene Symbol nNOS
Gene ID (NCBI) 4842
RRIDAB_10598169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29475
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1). The antibody can recognize isoform 1,2,4 of nNOS.