Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, T-47D cells, human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
21903-1-AP targets Nectin-4/PVRL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Nectin-4/PVRL4 fusion protein Ag14127 相同性解析による交差性が予測される生物種 |
| Full Name | poliovirus receptor-related 4 |
| Calculated molecular weight | 510 aa, 55 kDa |
| Observed molecular weight | 55-80 kDa, 43 kDa, |
| GenBank accession number | BC010423 |
| Gene Symbol | Nectin-4 |
| Gene ID (NCBI) | 81607 |
| RRID | AB_2878941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a glycosylated form of 60-80 kDa and a deglycosylated form of 55-70 kDa. The 43 kDa band immunoprecipitated in the supernatant corresponds to the soluble Nectin-4 form. (PMID: 15784625).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nectin-4/PVRL4 antibody 21903-1-AP | Download protocol |
| IHC protocol for Nectin-4/PVRL4 antibody 21903-1-AP | Download protocol |
| IF protocol for Nectin-4/PVRL4 antibody 21903-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Oncol MiR-31 and miR-128 regulates poliovirus receptor-related 4 mediated measles virus infectivity in tumors. | ||
Int J Urol Chemotherapy with gemcitabine and cisplatin downregulates tumor expression level of nectin-4 in a syngeneic model of murine MBT2 urothelial cancer cell line and C3H mice | ||
Oncol Lett Upregulation of nectin-4 is associated with ITGB1 and vasculogenic mimicry and may serve as a predictor of poor prognosis in colorectal cancer. | ||
Cell Biosci Interferon-stimulated gene PVRL4 broadly suppresses viral entry by inhibiting viral-cellular membrane fusion
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