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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Nectin-4/PVRL4 Monoclonal antibody (67721-1-Ig)

    WB analysis of human placenta using 67721-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67721-1-Ig (Nectin-4/PVRL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Nectin-4/PVRL4 Monoclonal antibody (67721-1-Ig)

    WB analysis of A431 using 67721-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67721-1-Ig (Nectin-4/PVRL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Nectin-4/PVRL4 Monoclonal antibody

Nectin-4/PVRL4 Monoclonal Antibody for WB, ELISA
Cat No. 67721-1-Ig
Clone No.2D4B6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin-4, poliovirus receptor related 4, PRR4, PVRL4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman placenta tissue, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67721-1-Ig targets Nectin-4/PVRL4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nectin-4/PVRL4 fusion protein Ag14414 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor-related 4
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight43 kDa
GenBank accession numberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
RRIDAB_2882908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96NY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

Protocols

Product Specific Protocols
WB protocol for Nectin-4/PVRL4 antibody 67721-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols