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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

nectin 4 Recombinant antibody, PBS Only (Capture)

nectin 4 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240009E4

Cat no : 83223-1-PBS

Synonyms

Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, LNIR

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00147-4

    Sandwich ELISA standard curve of MP00147-4

    Sandwich ELISA standard curve of MP00147-4, Human Nectin-4/PVRL4 Recombinant Matched Antibody Pair - PBS only. 83223-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0415. 83223-7-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/ml.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    Urine of eight individual healthy human donors was measured. The Nectin-4/PVRL4 concentration of detected samples was determined to be 3,920.62 pg/mL with a range of 249.12 - 9,633.39 pg/mL.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. 1 x 10^6 cells/mL were lysated by RIPA buffer, and the supernate was assayed and measured 3.2 ng/mL.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    The mean Nectin-4/PVRL4 concentration was determined to be 102.1 ng/mL in MDA-MB-468 cell extract based on a 3.1 mg/mL extract load and 107.4 ng/mL in MCF-7 cell extract based on a 4.2 mg/mL extract load.

  • Sample test of MP00147-4

    Sample test of MP00147-4

    Serum of sixteen individual healthy human donors was measured. The Nectin-4/PVRL4 concentration of detected samples was determined to be 750.17 pg/mL with a range of 392.48 - 1,810.23 pg/mL.

  • Cytometric bead array standard curve of MP00147-1

    Cytometric bead array standard curve of MP00147-1

    Cytometric bead array standard curve of MP00147-1, nectin 4 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83223-1-PBS. Detection antibody: 83223-4-PBS. Standard: Eg0415. Range: 0.625-10 ng/mL.

  • Cytometric bead array standard curve of MP00147-2

    Cytometric bead array standard curve of MP00147-2

    Cytometric bead array standard curve of MP00147-2, nectin 4 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83223-1-PBS. Detection antibody: 83223-3-PBS. Standard: Eg0415. Range: 0.625-10 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83223-1-PBS targets nectin 4 as part of a matched antibody pair:

MP00147-1: 83223-1-PBS capture and 83223-4-PBS detection (validated in Cytometric bead array)

MP00147-2: 83223-1-PBS capture and 83223-3-PBS detection (validated in Cytometric bead array)

MP00147-4: 83223-1-PBS capture and 83223-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=8.49 x 10-10M
KOff=2.20 x 10-4M
KOn=2.59 x 105M
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor-related 4
Calculated molecular weight 510 aa, 55 kDa
GenBank accession numberBC010423
Gene symbol Nectin-4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).