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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

p130Cas / BCAR1 Monoclonal antibody, PBS Only

p130Cas / BCAR1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D11C10

Cat no : 67215-1-PBS

Synonyms

BCAR1, 2D11C10, CASS1, CAS1, CAS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig)

    FC experiment of A431 using 67215-1-Ig (same clone as 67215-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig, Clone:2D11C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67215-1-PBS targets p130Cas / BCAR1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen p130Cas / BCAR1 fusion protein Ag10604 相同性解析による交差性が予測される生物種
Full Name breast cancer anti-estrogen resistance 1
Calculated molecular weight 870 aa, 93 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC062556
Gene symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_2882507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.