SNIP/p140Cap Polyclonal antibody

SNIP/p140Cap Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 55362-1-AP

Synonyms

SNIP, SNAP25 interacting protein, SNAP 25 interacting protein, p140Cap, P140


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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55362-1-AP targets SNIP/p140Cap in WB, IHC, IP, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SNAP25-interacting protein
Calculated molecular weight 127 kDa
Observed molecular weight 140-145 kDa
GenBank accession numberNM_025248
Gene symbol SNIP
Gene ID (NCBI) 80725
RRIDAB_2881315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SNIP/p140Cap antibody 55362-1-APDownload protocol
IHC protocol for SNIP/p140Cap antibody 55362-1-APDownload protocol
IP protocol for SNIP/p140Cap antibody 55362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

EBioMedicine

Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasis

Authors - Wen Wen Xu
mouseWB

Cell Rep

The kinesin Kif21b binds myosin Va and mediates changes in actin dynamics underlying homeostatic synaptic downscaling

Authors - Kira V Gromova
mouseWB

Mol Neurobiol

SNX17 Mediates Dendritic Spine Maturation via p140Cap

Authors - Qiuyan Cui