SNIP/p140Cap Polyclonal antibody
SNIP/p140Cap Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat and More (1)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 55362-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
Product Information
55362-1-AP targets SNIP/p140Cap in WB, IHC, IP, ELISA applications and shows reactivity with mouse, rat samples.
Tested Reactivity | mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | SNAP25-interacting protein |
Calculated molecular weight | 127 kDa |
Observed molecular weight | 140-145 kDa |
GenBank accession number | NM_025248 |
Gene symbol | SNIP |
Gene ID (NCBI) | 80725 |
RRID | AB_2881315 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SNIP/p140Cap antibody 55362-1-AP | Download protocol |
IHC protocol for SNIP/p140Cap antibody 55362-1-AP | Download protocol |
IP protocol for SNIP/p140Cap antibody 55362-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EBioMedicine Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasis | ||
Cell Rep The kinesin Kif21b binds myosin Va and mediates changes in actin dynamics underlying homeostatic synaptic downscaling | ||