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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

p14ARF/p19ARF Polyclonal antibody

p14ARF/p19ARF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31221-1-AP

Synonyms

p14ARF, p19ARF, CDKN2A, MTS1, P16-INK4A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p14ARF/p19ARF Polyclonal antibody (31221-1-AP)

    WB analysis using 31221-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31221-1-AP (p14ARF/p19ARF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using p14ARF/p19ARF Polyclonal antibody (31221-1-AP)

    IHC staining of human ovary cancer using 31221-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31221-1-AP (p14ARF/p19ARF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using p14ARF/p19ARF Polyclonal antibody (31221-1-AP)

    IF Staining of HeLa using 31221-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P16-INK4A antibody (31221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31221-1-AP targets p14ARF/p19ARF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p14ARF/p19ARF fusion protein Ag33340 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
Calculated molecular weight14kd
Observed molecular weight 19 kDa
GenBank accession numberNM_058195
Gene symbol P16-INK4A
Gene ID (NCBI) 1029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDKN2A locus on chromosome 9p21 encodes two transcripts/proteins INK4A and ARF. INK4A (also referred to as p16INK4A) specially blocks the CDK4 and CDK6 cyclin-dependent kinases that control the phosphorylation status of the retinoblastoma (RB) protein (PMID: 11584300, PMID: 8259215). ARF (known as p14ARF in man and p19ARF in mouse) has been designed as a positive regulator of p53 levels because, through its complexation with Mdm2 (HDM2 in human), it prevents mdm2-mediated cytoplasmic translocation and degradation of p53 (PMID: 9153396).

Protocols

Product Specific Protocols
WB protocol for p14ARF/p19ARF antibody 31221-1-APDownload protocol
IHC protocol for p14ARF/p19ARF antibody 31221-1-APDownload protocol
IF protocol for p14ARF/p19ARF antibody 31221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols