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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

p16INK4a Polyclonal antibody

p16INK4a Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28416-1-AP

Synonyms

Cdkn2a, p16-INK4a, p16-INK4, p16(INK4a), p16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDKN2A/p19ARF Polyclonal antibody (28416-1-AP)

    WB analysis using 28416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (Cdkn2a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using p16INK4a Polyclonal antibody (28416-1-AP)

    WB analysis using 28416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (p16INK4a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inNIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse uterus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28416-1-AP targets p16INK4a in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p16INK4a fusion protein Ag28927 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 2A
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberNM_001040654
Gene symbol Cdkn2a
Gene ID (NCBI) 12578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cdkn2a (cyclin-dependent kinase inhibitor 2a) is a cell cycle regulating gene encoding alternate proteins, p16Ink4a and p19Arf, that arrest the G1 phase through CDK4/6 inhibition. As they are tumor suppressors, p16Ink4a and p19Arf can be targeted for treatment of various cancers, including melanoma, cervical, and esophageal cancers.

Protocols

Product Specific Protocols
WB protocol for p16INK4a antibody 28416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols