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  • Featured Product
  • KD/KO Validated

p38 MAPK Monoclonal antibody, PBS Only (Capture)

p38 MAPK Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1C2

Cat no : 66234-1-PBS

Synonyms

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using p38 MAPK Monoclonal antibody (66234-1-Ig)

    WB analysis of HEK-293 using 66234-1-Ig (same clone as 66234-1-PBS)

    WB result of p38 MAPK antibody (66234-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 MAPK transfected HEK-293 cells. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using p38 MAPK Monoclonal antibody (66234-1-Ig)

    WB analysis of human heart using 66234-1-Ig (same clone as 66234-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using p38 MAPK Monoclonal antibody (66234-1-Ig)

    WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using p38 MAPK Monoclonal antibody (66234-1-Ig)

    WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using p38 MAPK Monoclonal antibody (66234-1-Ig)

    WB analysis of pig heart using 66234-1-Ig (same clone as 66234-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using p38 MAPK Monoclonal antibody (66234-1-Ig)

    IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using p38 MAPK Monoclonal antibody (66234-1-Ig)

    IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using p38 MAPK Monoclonal antibody (66234-1-Ig)

    IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using p38 MAPK Monoclonal antibody (66234-1-Ig)

    IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using p38 MAPK Monoclonal antibody (66234-1-Ig)

    IHC staining of human gliomas using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using p38 MAPK Monoclonal antibody (66234-1-Ig)

    IHC staining of human gliomas using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using p38 MAPK Monoclonal antibody (66234-1-Ig)

    IF Staining of HepG2 using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p38 MAPK antibody (66234-1-Ig, Clone: 1A1C2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50419-1

    Cytometric bead array standard curve of MP50419-1

    Cytometric bead array standard curve of MP50419-1, p38 MAPK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66234-1-PBS. Detection antibody: 68965-1-PBS. Standard:Ag5797. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP50419-2

    Cytometric bead array standard curve of MP50419-2

    Cytometric bead array standard curve of MP50419-2, p38 MAPK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66234-1-PBS. Detection antibody: 68966-1-PBS. Standard:Ag5797. Range: 1.563-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66234-1-PBS targets p38 MAPK as part of a matched antibody pair:

MP50419-1: 66234-1-PBS capture and 68965-1-PBS detection (validated in Cytometric bead array)

MP50419-2: 66234-1-PBS capture and 68966-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen p38 MAPK fusion protein Ag5797 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 14
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC031574
Gene symbol p38 MAPK
Gene ID (NCBI) 1432
RRIDAB_2861335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.