Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells, Jurkat cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
23317-1-AP targets p57Kip2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | p57Kip2 fusion protein Ag19849 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin-dependent kinase inhibitor 1C (p57, Kip2) |
| Calculated molecular weight | 316 aa, 32 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC067842 |
| Gene Symbol | p57 Kip2/CDKN1C |
| Gene ID (NCBI) | 1028 |
| RRID | AB_2879254 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49918 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p57Kip2 antibody 23317-1-AP | Download protocol |
| IHC protocol for p57Kip2 antibody 23317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Cancer Res microRNA-221 Enhances MYCN via Targeting Nemo-like Kinase and Functions as an Oncogene Related to Poor Prognosis in Neuroblastoma. | ||
FASEB J Downregulation of ALKBH5 rejuvenates aged human mesenchymal stem cells and enhances their therapeutic efficacy in myocardial infarction | ||
Cancer Med PIK3R3 is upregulated in liver cancer and activates Akt signaling to control cancer growth by regulation of CDKN1C and SMC1A
| ||
Cell Death Dis The FOXM1/RNF26/p57 axis regulates the cell cycle to promote the aggressiveness of bladder cancer. | ||
Oncogene Dual network analysis of transcriptome data for discovery of new therapeutic targets in non-small cell lung cancer | ||