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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

p57Kip2 Monoclonal antibody, PBS Only

p57Kip2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12H3

Cat no : 66794-1-PBS

Synonyms

CDKN1C, KIP2, p57, Cyclin-dependent kinase inhibitor p57, Cyclin-dependent kinase inhibitor 1C

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using p57Kip2 Monoclonal antibody (66794-1-Ig)

    WB analysis using 66794-1-Ig (same clone as 66794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66794-1-Ig (CDKN1C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using p57Kip2 Monoclonal antibody (66794-1-Ig)

    WB analysis using 66794-1-Ig (same clone as 66794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using p57Kip2 Monoclonal antibody (66794-1-Ig)

    WB analysis of HeLa using 66794-1-Ig (same clone as 66794-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66794-1-Ig (CDKN1C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using p57Kip2 Monoclonal antibody (66794-1-Ig)

    IHC staining of human placenta using 66794-1-Ig (same clone as 66794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using p57Kip2 Monoclonal antibody (66794-1-Ig)

    IHC staining of human placenta using 66794-1-Ig (same clone as 66794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66794-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66794-1-PBS targets p57Kip2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen p57Kip2 fusion protein Ag19927 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase inhibitor 1C (p57, Kip2)
Calculated molecular weight 316 aa, 32 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC067842
Gene symbol p57 Kip2/CDKN1C
Gene ID (NCBI) 1028
RRIDAB_2882138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.