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NF-κB p65 抗体 708 文献

Rabbit Polyclonal| カタログ番号: 10745-1-AP

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA
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Images See all

NF-κB p65 Antibody WB HEK-293 cells 10745-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65; RELA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NF-κB p65 Antibody WB Hek-293 cells 10745-1-AP

WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

NF-κB p65 Antibody WB HEK-293 cells 10745-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NF-κB p65 Antibody IP HeLa cells 10745-1-AP

IP Result of anti-p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

NF-κB p65 Antibody IHC human breast cancer tissue 10745-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10745-1-AP (p65 antibody) at dilution of 1:100 (under 40x lens).

NF-κB p65 Antibody IF HepG2 cells 10745-1-AP

Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

NF-κB p65 Antibody FC HeLa cells 10745-1-AP

1X10^6 HeLa cells were stained with 0.2ug p65; RELA antibody (10745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

種特異性:
human, mouse, rat, Artemisia sphaerocephala Krasch, Bombyx mori, bovine, canine, CHICKEN, fish, hamster, monkey
WB ポジティブ:
HEK-293 cells, A431 cells, Jurkat cells, MCF-7 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, HeLa cells, mouse lung tissue, rat lung tissue, mouse kidney tissue, rat kidney tissue
IP ポジティブ:
HeLa cells
IHC ポジティブ:
human breast cancer tissue, human liver cancer tissue, human stomach tissue, mouse heart tissue, mouse kidney tissue
IF ポジティブ:
HepG2 cells
FC ポジティブ:
HeLa cells
推奨希釈倍率:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
NF-κB p65 fusion protein Ag1199
正式名:
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
計算分子量:
65 kDa
検出分子量:
65 kDa
GenBank accession number:
BC011603
Gene ID (NCBI):
5970
遺伝子シンボル:
RELA
別名:
NF Kappa B, NF κB, NFKB 3, NFKB p65, NFKB3, NF-κB, NF-κB3, p65, RELA, Transcription factor p65

背景説明

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.


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