ChromoTek pSpot4 vector
The plasmid pSpot4 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot4, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
Cat no : ev-4
Synonyms
Validation Data Gallery
Product Information
The plasmid pSpot4 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot4, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
| Applications | Protein expression |
| Vector type | bacterial expression vector |
| Promoter | T7 |
| Replication | pBR322 |
| Reporter | Spot-Tag® (PDRVRAVSHWSS) N-terminal |
| Selection | ampicillin |