ChromoTek pSpot5 vector
The plasmid pSpot5 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot5, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
Vector type
yeast expression vector
Promoter
TPI1
Reporter
Spot-Tag® N-terminal
Cat no : ev-5
Synonyms
Validation Data Gallery
Product Information
The plasmid pSpot5 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot5, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
| Applications | Protein expression |
| Vector type | yeast expression vector |
| Promoter | TPI1 |
| Replication | CEN (S. cerevisiae) pBR322 (E. coli) |
| Reporter | Spot-Tag® (PDRVRAVSHWSS) N-terminal |
| Selection | leucin (S. cerevisiae) Ampicillin (E. coli)6 |
Publications
| Application | Title |
|---|---|
Life Sci Alliance The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair. |