ChromoTek pSpot7 vector
The plasmid pSpot7 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot7, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
Cat no : ev-7
Synonyms
Validation Data Gallery
Product Information
The plasmid pSpot7 is an expression vector for Spot-Tag® fusion proteins in S. cerevisiae. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot7, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the N-terminus of the POI.
| Applications | Protein expression |
| Vector type | yeast expression vector |
| Promoter | TPI1 |
| Replication | 2µ (S. cerevisiae) pBR322 (E. coli) |
| Reporter | Spot-Tag® (PDRVRAVSHWSS) N-terminal |
| Selection | leucin (S. cerevisiae) ampicillin (E. coli) |