ChromoTek pSpot8 GFP-Spot-Tag
The plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.
Cat no : ev-33
Synonyms
Validation Data Gallery
Product Information
The plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system.
| Description | The plasmid pSpot8_GFP-Spot-Tag is a S. cerevisiae expression vector encoding the Aequorea victoria Green fluorescent protein (GFP) variant GFPuv fused to the Spot-Tag®. The Spot-Tag® comprises the sequence PDRVRAVSHWSS and is fused to the C-terminus of GFPuv. This plasmid may be used as a positive control for immunoprecipitation or Western blot experiments using the Spot-Tag® system. |
| Applications | Protein expression |
| Vector type | Yeast expression vector |
| Promoter | TPI1 |
| Replication | 2µ (S. cerevisiae) pBR322 (E. coli) |
| Reporter | Spot-Tag® (PDRVRAVSHWSS) C-termina |
| Selection | Leucin (S. cerevisiae) Ampicillin (E. coli) |