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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

pan-RAS Recombinant antibody

pan-RAS Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2I22

Cat no : 81615-1-RR

Synonyms

KRAS, KRAS4A, KRAS4B, pan RAS, EC:3.6.5.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using pan-RAS Recombinant antibody (81615-1-RR)

    WB analysis using 81615-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81615-1-RR (pan-RAS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using pan-RAS Recombinant antibody (81615-1-RR)

    IHC staining of human colon cancer using 81615-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using pan-RAS Recombinant antibody (81615-1-RR)

    IHC staining of human colon cancer using 81615-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using pan-RAS Recombinant antibody (81615-1-RR)

    IF Staining of HeLa using 81615-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using pan-RAS antibody (81615-1-RR, Clone: 2I22 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81615-1-RR

    Affinity and Kinetic Characterization of 81615-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81615-1-RR against Human pan-RAS were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, mouse brain tissue, rat brain tissue, pig liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81615-1-RR targets pan-RAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen pan-RAS fusion protein Ag2700 相同性解析による交差性が予測される生物種
Full Name v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight 188 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC013572
Gene symbol KRAS
Gene ID (NCBI) 3845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Protocols

Product Specific Protocols
WB protocol for pan-RAS antibody 81615-1-RRDownload protocol
IHC protocol for pan-RAS antibody 81615-1-RRDownload protocol
IF protocol for pan-RAS antibody 81615-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols