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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    WB analysis of HepG2 using 60202-1-Ig (same clone as 60202-1-PBS)

    WB result of peroxiredoxin 2 antibody (60202-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    WB analysis using 60202-1-Ig (same clone as 60202-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    WB analysis of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    WB analysis of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    WB analysis of fetal human brain using 60202-1-Ig (same clone as 60202-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    WB analysis of HEK-293 using 60202-1-Ig (same clone as 60202-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    IHC staining of human brain using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    IHC staining of human brain using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (peroxiredoxin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using peroxiredoxin 2 Monoclonal antibody (60202-1-Ig)

    IF Staining of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using peroxiredoxin 2 antibody (60202-1-Ig, Clone: 3H6C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

peroxiredoxin 2 Monoclonal antibody, PBS Only

peroxiredoxin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60202-1-PBS
Clone No.3H6C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60202-1-PBS targets peroxiredoxin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen peroxiredoxin 2 fusion protein Ag0835 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC003022
Gene Symbol peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_10918978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32119
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)