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Phospho-TDP43 (Ser409/410) Recombinant antibody

Phospho-TDP43 (Ser409/410) Recombinant Antibody for WB, IHC, ELISA
Cat No. 80007-1-RR
Clone No.6M10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, Calyculin A treated cells
Positive IHC detected infrontal cortex of FTLD-TDP type B case, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80007-1-RR targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_007375
Gene Symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Protocols

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 80007-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

Regulation of TAR DNA binding protein 43 (TDP-43) homeostasis by cytosolic DNA accumulation

Authors - Cha Yang
WB

Biochim Biophys Acta Mol Basis Dis

Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition.

Authors - Josephine C Esposto
humanWB,IF

bioRxiv

TDP-43 pathology links innate and adaptive immunity in amyotrophic lateral sclerosis

Authors - Baggio A Evangelista
mouseIF

Res Sq

Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence

Authors - Joy Mitra
mouseWB

Mol Neurobiol

A Panel of miRNA Biomarkers Common to Serum and Brain-Derived Extracellular Vesicles Identified in Mouse Model of Amyotrophic Lateral Sclerosis

Authors - Natasha Vassileff
mouseIF

J Exp Med

A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice

Authors - David Brenner
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