Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Calyculin A treated cells |
Positive IHC detected in | frontal cortex of FTLD-TDP type B case, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 5 publications below |
IHC | See 7 publications below |
IF | See 7 publications below |
Product Information
80007-1-RR targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | TAR DNA binding protein |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | NM_007375 |
Gene Symbol | TDP-43 |
Gene ID (NCBI) | 23435 |
RRID | AB_2882937 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13148 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-TDP43 (Ser409/410) antibody 80007-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem Regulation of TAR DNA binding protein 43 (TDP-43) homeostasis by cytosolic DNA accumulation | ||
Biochim Biophys Acta Mol Basis Dis Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition. | ||
bioRxiv TDP-43 pathology links innate and adaptive immunity in amyotrophic lateral sclerosis | ||
Res Sq Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence | ||
Mol Neurobiol A Panel of miRNA Biomarkers Common to Serum and Brain-Derived Extracellular Vesicles Identified in Mouse Model of Amyotrophic Lateral Sclerosis | ||
J Exp Med A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice |