Phospho-AKT (Tyr315) Monoclonal antibody
Phospho-Akt (Tyr315) Monoclonal Antibody for ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5C4D12
Cat no : 60072-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 11 publications below |
IF | See 1 publications below |
Product Information
60072-1-Ig targets Phospho-Akt (Tyr315) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Phospho Peptide 相同性解析による交差性が予測される生物種 |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Calculated molecular weight | 56 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | NM_005163 |
Gene symbol | AKT1 |
Gene ID (NCBI) | 207 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein kinase B (PKB/Akt) has been well established as an important signaling intermediate, and its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337). Akt, also known as protein kinase B (PKB), is a serine/threonine kinase activated downstream of class I phosphatidylinositol 3-kinase (PI3K) and various receptors. It has emerged as the key regulator for many signal transduction pathways, modulating multiple processes such as cell survival, proliferation, growth, angiogenesis, and glucose metabolism(PMID:16139227). Its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337).
Publications
Species | Application | Title |
---|---|---|
Sci Rep TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis. | ||
Neuropharmacology Puerarin protected the brain from cerebral ischemia injury via astrocyte apoptosis inhibition. | ||
Mar Drugs 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways. | ||
PLoS One Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters. | ||
Apoptosis SZC017, a novel oleanolic acid derivative, induces apoptosis and autophagy in human breast cancer cells. | ||
Biochim Biophys Acta Nuclear PTEN interferes with binding of Ku70 at double-strand breaks through post-translational poly(ADP-ribosyl)ation. |