Phospho-Marcksl1 Polyclonal antibody
Phospho-Marcksl1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse and More (3)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 10018-3-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
10018-3-AP targets Phospho-Marcksl1 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | myristoylated alanine rich protein kinase C substrate |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC046601 |
| Gene symbol | Marcks |
| Gene ID (NCBI) | 17118 |
| RRID | AB_2297345 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated. Because of the sequence similarity, this antibody can recognize phospho-Marcksl1 and phospho-Marcks.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Marcksl1 antibody 10018-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability. | ||
J Neurosci NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia. | ||
J Physiol 5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase. | ||
Am J Physiol Cell Physiol Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability. | ||
