• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human CNTF protein

Activity

2.75-14 ng/mL

Species Reactivity

human

Purity

>95%

Cat no : HZ-1331

Synonyms

Ciliary Neurotrophic Factor



Technical Specifications

GeneID 1270
Species Human
Expression HEK293
Activity 2.75-14 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP26441
Molecular Mass 23-27 kDa reduced and non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vials to mix.

Background

CNTF or ciliary neurotrophic factor, is a member of the IL-6 family of cytokines alongside LIF, OSM, and cardiotropin-1. It promotes gene expression, cell survival, and differentiation of sensory, ciliary, and motor neurons. It promotes rod and cone photoreceptor regeneration in response to retinal injury and degeneration. It is also thought to control B cell differentiation in the bone marrow. CNTF can be used to maintain neural stem cells in the pluripotent state, or it can promote their further differentiation into glial precursor cells, astrocytes, and oligodendrocytes (PMID: 30504707, 21912637, 26487326).

Synonyms

Ciliary Neurotrophic Factor

Publications

SpeciesTitle

ACS Biomater Sci Eng

Electrospun PCL Nerve Conduit Filled with GelMA Gel for CNTF and IGF-1 Delivery in Promoting Sciatic Nerve Regeneration in Rat

Authors - Hailiang Xu