• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human DKK-1 protein

Activity

7-70 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1314

Synonyms

SK, Dickkopf-1, Dickkopf-related protein-1, mDkk-1



Technical Specifications

GeneID 22943
Species Human
Expression HEK293
Activity 7-70 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberO94907
Molecular Mass 37-51 kDa reduced, 35-49 non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

DKK-1 (Dickkopf WNT signaling pathway inhibitor 1) is a member of the DKK protein family. Other members of this family include DKK-2, DKK-3, and DKK-4. The DKK family proteins share 35-40 % homology between various members. Recombinant human DKK-1 expressed in HEK293 cells is a 35-45kDa glycosylated protein. It is a secreted protein that functions as a negative regulator of Wnt/β-catenin signaling pathway. Wnt/ β-catenin signaling pathway involves binding of the Wnt ligands to the seven-transmembrane receptor, Frizzled, and the coreceptor lipoprotein-related protein 5 and 6 (LRP5/6). DKK1 forms a ternary complex with LRP5/6 and another receptor, Kremen, followed by endocytosis of this complex and removal of LRP5/6 from the cell surface. It has been suggested that DKK-1 permits anterior development by inhibiting Wnt/ β-catenin signaling, which is essential for posterior patterning in vertebrates. The balance between Wnt signaling and DKK-1 inhibition is critical for bone formation and homeostasis and insufficient or excess DKK-1 activity in bone results in increased or decreased bone density, respectively. (PMID: 17143291; 12050670; 10570958)

Synonyms

SK, Dickkopf-1, Dickkopf-related protein-1, mDkk-1

Publications

SpeciesTitle
human

Cell Stem Cell

Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro.

Authors - Anindita Sarkar