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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-19 protein

Activity

50-300 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1330

Synonyms

FGF-19, FGF19, FGFJ, Fibroblast Growth Factor-19

Validation Data Gallery

  • Purity of recombinant human FGF-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human FGF-19 (HZ-1330) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-19 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 50-300 ng/mL.

    Recombinant human FGF-19 (HZ-1330) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-19 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 50-300 ng/mL.

  • HepG2 cells were serum starved overnight, and treated with FGF-19 (HZ-1330) for 30 min at indicated concentrations. Cells were harvested and lysed using RIPA buffer with inhibitors. Western blot was performed with Proteintech antibodies, phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody (28733-1-AP) and ERK1/2 Polyclonal antibody (11257-1-AP).

    HepG2 cells were serum starved overnight, and treated with FGF-19 (HZ-1330) for 30 min at indicated concentrations. Cells were harvested and lysed using RIPA buffer with inhibitors. Western blot was performed with Proteintech antibodies, phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody (28733-1-AP) and ERK1/2 Polyclonal antibody (11257-1-AP).

Technical Specifications

GeneID 9965
Species Human
Expression HEK293
Activity 50-300 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberO95750
Molecular Mass 23 kDa reduced, 22 kDa non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

FGF-19 is a member of the endocrine subfamily of FGFs. It primarily binds and activates signaling pathways downstream of FGFR4 in the liver. Through downregulation of the CYP7A1 enzyme, FGF-19 can prevent overproduction of bile salts in the liver and contribute towards their homeostasis. It is also a regulator of glucose and lipid metabolism alongside FGF-21. FGF-19 can additionally stimulate hepatocyte proliferation and has been correlated with the progression of hepatocellular carcinoma when its signaling levels are increased. (PMID: 30191047, 21437243, 20660733).

Synonyms

FGF-19, FGF19, FGFJ, Fibroblast Growth Factor-19

Publications

SpeciesTitle
mouse

Cell Metab

Hyodeoxycholic acid alleviates non-alcoholic fatty liver disease through modulating the gut-liver axis

Authors - Junliang Kuang
View Article