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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-9 protein

Activity

2-11 ng/mL EC50

Species Reactivity

monkey,human

Purity

>95%

Cat no : HZ-1329

Synonyms

Glia-activating factor (GAF) Heparin-binding growth factor 9 (HBGF-9) Fibroblast Growth Factor-9

Validation Data Gallery

  • Purity of recombinant human FGF-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FGF-9 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human FGF-9 (HZ-1329) induces dose-dependent proliferation of the 4MBr-5 (monkey epithelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant FGF-9 for 7 days. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 4-20 ng/mL.

    Recombinant human FGF-9 (HZ-1329) induces dose-dependent proliferation of the 4MBr-5 (monkey epithelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant FGF-9 for 7 days. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 4-20 ng/mL.

Technical Specifications

GeneID 2254
Species Human
Expression HEK293
Activity 2-11 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP31371
Molecular Mass 25 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity monkey,human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

FGF-9 is a 26 kdA, glycosylated protein that is a member of the FGF superfamily. It primarily binds with FGFR3 and plays prominent roles in the regulating embryonic, lung, and skeletal development. It is a key regulator of male sex determination through promoting the proliferation of pre-Sertoli cells. It signals in conjunction with FGF-10 and SHH to drive the development of the lung mesenchymal space. FGF-9 is also a positive regulator of chondrocyte proliferation and osteogenesis during the bone formation process. Overexpression of loss of FGF-9 during skeletal development often results in aberrant bone growth. (PMID: 28395336, 25772309, 16540513, 25435023).

Synonyms

Glia-activating factor (GAF) Heparin-binding growth factor 9 (HBGF-9) Fibroblast Growth Factor-9