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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IGF-I protein- GMP grade

EC50

2-14 ng/mL

Specific Activity

minimally 3.00 x 102 IU/mg, typically 6.94 x 102 IU/mg

Species Reactivity

human

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1322-GMP

Synonyms

H-IGF-1, IGF, IGF-I, IGF-IA, IGF-IB, IGF1A, IGFIa, Insulin like growth factor, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, M-IGF-1, Mechano growth factor, MGF, OTTHUMP00000195084, R-IGF-1, Somatomedin C, Somatomedin-C

Validation Data Gallery

  • Purity of recombinant human IL-12 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-12 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP recombinant human IGF-I (Cat no: HZ-1322-GMP) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 2-14 ng/mL.

    GMP recombinant human IGF-I (Cat no: HZ-1322-GMP) stimulates dose-dependent proliferation of the MCF-7 human breast cancer cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MCF-7 cells were treated with increasing concentrations of recombinant IGF-I for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 2-14 ng/mL.

Technical Specifications

GeneID 3479
Species Human
Expression HEK293
EC50 2-14 ng/mL
Specific Activityminimally 3.00 x 102 IU/mg, typically 6.94 x 102 IU/mg
Purity > 95%
Endotoxin< 0.1 EU/ug
Accession NumberP05019
Molecular Mass 9-10 kDa reduced and non-reduced, monomer, non-glycosylated
Formulation 50 mM Acetate pH 4.0
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

The Insulin like growth factor-1, also known as Somatomedin-C is a growth factor which is structurally related to insulin and is an important regulator of growth and differentiation in various tissues and cell systems. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptide. The two precursor isoforms are differentially expressed by various tissues. The proteolytic cleavage of the N- and C-terminal regions results in the mature IGF-1 protein which is identical between isoforms. IGF-1 binds to IGF-1 receptor and induces receptor autophosphorylation. This further phosphorylates Insulin receptor substrate -1 (IRS-1) and activates various downstream signaling pathways including the PI3-AKT, MAPK etc. (PMID: 17354613, 17113337, 29535161)

Synonyms

H-IGF-1, IGF, IGF-I, IGF-IA, IGF-IB, IGF1A, IGFIa, Insulin like growth factor, insulin-like growth factor 1 (somatomedin C), Insulin-like growth factor I, insulin-like growth factor IB, M-IGF-1, Mechano growth factor, MGF, OTTHUMP00000195084, R-IGF-1, Somatomedin C, Somatomedin-C