• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-15 protein

Activity

0.07-0.37 ng/mL EC50 for NK-92 bioassay.

Specific Activity

>4.00 x 107 IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Cat no : HZ-1323

Synonyms

Interleukin 15, IL15, IL-15MGC9721, Interleukin-15



Technical Specifications

GeneID 3600
Species Human
Expression HEK293
Activity 0.07-0.37 ng/mL EC50 for NK-92 bioassay.
Specific Activity>4.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP40933
Molecular Mass 12 to 24 kDa reduced, 11 to 23 kDa non-reduced, monomer, glycosylated
Formulation 50 mM Acetate pH 4.0 + 150mM NaCl
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-15 is a 4-α-helix bundle cytokine playing a pivotal role in stimulation of both innate and adaptive immune cells. It is produced primarily by keratinocytes, skeletal muscle cells, monocytes, and CD4+ T cells. It is a member of the common gamma chain family. The members of the common gamma chain family include the IL-2, IL-4, IL-7, IL-9, and IL-21 and require binding to the common  chain receptor for activation. It can be used in growth and maintenance of T and NK cells. It is also shown to be used in proliferation and functional effect of T cells for adoptive cell therapy. It is a glycosylated protein and HumanKine IL-15 appear as 12.5-25 kDa bands (PMID: 24587813, 26627006, 27849617, 31250350)

Synonyms

Interleukin 15, IL15, IL-15MGC9721, Interleukin-15

Publications

SpeciesTitle
human

Front Oncol

Combination of 4-1BB and DAP10 promotes proliferation and persistence of NKG2D(bbz) CAR-T cells

Authors - Cheng Wei
mouse

Nat Commun

Early life gut microbiota sustains liver-resident natural killer cells maturation via the butyrate-IL-18 axis

Authors - Panpan Tian
rat

Heliyon

Astragalus regulates the intestinal immune response during sepsis by mediating ILC3 proliferation through RORγt

Authors - Jin Li