- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IL-21 protein- GMP grade
EC50
0.25-1.25 ng/mL
Species Reactivity
human
Purity
>95%
Cat no : HZ-1319-GMP
Synonyms
Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, CVID11
Validation Data Gallery
Technical Specifications
GeneID | 59067 |
Species | Human |
Expression | HEK293 |
EC50 | 0.25-1.25 ng/mL |
Purity | >95% |
Endotoxin | <0.1 EU/μg |
Accession Number | Q9HBE4 |
Molecular Mass | 18 kDa reduced, 17 kDa non-reduced, monomer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human |
Stability and Reconstitution
Stability and Storage | Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
GMP Quality Policies
HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.
Background
IL-21 is a type I, 4-α-helix bundle cytokine primarily produced by CD4+ T cells and NK cells. It is a strong activator of proliferation and antibody production in B cells but does signal to numerous other immune cells as well. Elevated IL-21 signaling can promote several autoimmune diseases including type I diabetes, systemic lupus erythematosus (SLE), and rheumatoid arthritis (RA). However, it has also been shown to have potent antitumoral activity, making it a promising candidate for immunotherapy and as an adjuvant material in CAR-T cell therapy (PMID: 22235123, 26966515, 27577879).
Synonyms
Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, CVID11