• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-21 protein

Activity

0.25-1.25 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1319

Synonyms

Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, CVID11



Technical Specifications

GeneID 59067
Species Human
Expression HEK293
Activity 0.25-1.25 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ9HBE4
Molecular Mass 18 kDa reduced, 17 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-21 is a type I, 4-α-helix bundle cytokine primarily produced by CD4+ T cells and NK cells. It is a strong activator of proliferation and antibody production in B cells but does signal to numerous other immune cells as well. Elevated IL-21 signaling can promote several autoimmune diseases including type I diabetes, systemic lupus erythematosus (SLE), and rheumatoid arthritis (RA). However, it has also been shown to have potent antitumoral activity, making it a promising candidate for immunotherapy and as an adjuvant material in CAR-T cell therapy (PMID: 22235123, 26966515, 27577879).

Synonyms

Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, CVID11

Publications

SpeciesTitle

Immunity

Human microglia maturation is underpinned by specific gene regulatory networks

Authors - Claudia Z Han