- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human pro-IGF-II protein- GMP grade
EC50
12-80 ng/mL
Species Reactivity
human
Purity
>95%
Cat no : HZ-1161-GMP
Synonyms
C11orf43, FLJ22066, FLJ44734, IGF II, IGF2, INSIGF, Insulin like growth factor II, pp9974, Somatomedin A
Validation Data Gallery
Technical Specifications
| GeneID | 3481 |
| Species | Human |
| Expression | HEK293 |
| EC50 | 12-80 ng/mL |
| Purity | >95% |
| Endotoxin | <0.1 EU/µg |
| Accession Number | P01344 |
| Molecular Mass | 25 kDa reduced and non-reduced, monomer, glycosylated |
| Formulation | 10 mM Tris-HCl pH 7.4 + 350 mM NaCl, See Certificate of Analysis for details |
| Species Reactivity | human |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
GMP Quality Policies
HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.
Background
IGF2 is a hormone involved in the regulation of cell proliferation, growth, migration, differentiation, and survival. IGF2 is mostly expressed in early embryonic and fetal development. Adult IGF2 expression occurs in the liver and epithelial cells lining the surface of the brain. IGF2 can also be detected in plasma (PMID: 24593700; 23257688)
Synonyms
C11orf43, FLJ22066, FLJ44734, IGF II, IGF2, INSIGF, Insulin like growth factor II, pp9974, Somatomedin A